Pioneering
Oncogenetic
Diagnostics
in Georgia
ISO 15189 accredited. Next-generation sequencing(NGS), rtPCR,
Flow Cytometry, Sanger Sequencing
Our proud & happy partners
Services That
Transform The
Treatment Process
Innovative Technologies For Better Diagnostics
qPCR
QuantStudio 5 Real-Time PCR System
The QuantStudio 5 real-time PCR machine is a platform with cutting-edge, superior features and broad spectrum of working modes. This platform meets the Good Laboratory Practice (GLP) guide and other clinical standards requirements. There are listed some advantages of QuantStudio 5:
- High sensitivity and specificity – important for both clinical and scientific research.
- A sophisticated optical system ensures fluorescence detection with high accuracy and provides reliable results in quantitative and other types of nucleic acid analysis.
- Multiplexing capability – involves the simultaneous specific detection of up to 6 different targets in one reaction, which ensures the speed and efficiency of the analysis.
- A thermal block based on special technology provides constant, precise temperature control, which guarantees stable and reliable results.
- Maximum data security and traceability are also provided in the QuantStudio 5 PCR system.
Sanger Sequencing
SeqStudio Genetic Analyser
The SeqStudio Genetic Analyzer is a highly optimized platform of Sanger sequencing and fragment analysis, which is simple to apply for a wide range of experiments. The instrument uses an innovative cartridge that includes everything in one system, with a single polymer, compatible with both sequencing and fragment analysis experiments.
Advantages of the genetic analyzer:
- The SeqStudio genetic analyzer allows for individual cartridge design for different types of experiments;
- Different formats of results can be viewed on the screen and results can be easily evaluated;
- Through the Cloud system, the data from genetic analyzer is easily accessible to the operator at any time, via any Internet-connected computer, tablet, or mobile phone, which simplifies their management and monitoring.
Next Generation Sequencing (NGS)
Ion Chef & Ion GeneStudio S5
The Ion Chef system automates the process of NGS library preparation steps like: template preparation and loadingon the chip, which are some of the most important initial steps in Next-Generation Sequencing, ensuring standardization and high-speed performance. The instrument’s sophisticated visual system makes it easy to monitor the process during operation, and barcode recognition tool controls the expiration dates and correct placement of samples, chips, consumables, and reagents.
The Ion GeneStudio S5 system is a semiconductor-technology based next-generation sequencing (NGS) platform that enables targeted sequencing experiments, including genomic, exome, and transcriptomic studies. The S5 system uses the chips of Ion 5 series. Through automated filtering and analysis of results, high-quality sequencing results are obtained for both clinical and scientific use.
Major advantages:
- Standardization of experiments – reduced sample preparation time and complexity through cartridge-format reagents;
- Speed - The Ion GeneStudio S5 series instruments optimally integrate semiconductor sequencing technology, which allows providing high-quality sequencing data in a few hours. Complete reaction analysis is also performed in a few hours.’
- Small sample size requirement – Sequencing is complicated with the low amount of DNA sample, when the sample is difficult to obtain or formalin-fixed paraffin-embedded tissue specimens (FFPE) are applied, as well as in the case of extracellular DNA (cfDNA) samples. With Ion AmpliSeq technology and the Ion GeneStudio S5 series of instruments, it is possible to obtain informative results even with a small initial sample size.
- Simplicity of data analysis and archiving – Using Torrent Suite and Ion Reporter bioinformatics softwares, management and interpretation of large amounts of data is relatively simplified.
Flow Cytometry
Cytometer – NovoCyte
NovoCyte is a superior level flow cytometry platform designed to enable high-quality experiments in a variety of laboratories. The instrument enables detection of up to 17 parameters with high sensitivity and resolution. The cytometer’s versatility in terms of research objectives and specificity is derived from its ability to modify the laser and optic setup while maintaining the capabilities of complex cellular analysis.
Possible areas of analysis include:
- The study of apoptotic processes, which is facilitated by the NovoCyte flow cytometer through automatic adjustment of compensation parameters and the selection of a wide range of fluorescence detection modes;
- Immunophenotyping with the NovoCyte flow cytometer is performed in a multiplexed manner, with simultaneous analysis of many leukocytes, which allows the researcher/physician to determine the patient’s complete immune status and assess his/her response to an infectious agent;
- Cell proliferation can be assessed both by absolute cell quantification and by labeling with a suitable dye (CFSE), which is evenly distributed in newly formed cells after division;
- Detection of cytokines and their source of origin, which are the most important components of the immune response activated against different pathogens, by autoimmune reactions or therapeutic intervention, provides with an important information about gene regulation, immune response, and the course of inflammatory processes;
- Detection of intracellular proteins and (6) cell cycle analysis by labeling proteins and nucleic acids, respectively, are also experiments that can be performed with high accuracy and sensitivity using the flow cytometer described above.
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